Improve the quality of fluorescent images

One of the jobs done by using ImageJ is to enhance the contrast between the fluorescent actin filaments and dark background ( to make fluorescent filaments brighter, to make dark background darker).

The function used to achieve this is called 'contrast enhancer' as shown in Figure1.

Figure 1: Enhance Contrast

Meanwhile, an important assistant plugin used is called 'background subtractor' as shown in Figure2.

Another assistant function can be used to achieve our purpose is shown in Figure3. 

Figure 3: Assistant function

The processing results:

Figure 5: Before processing

Figure 6: After processing

Both of the fluorescent images record the motility of actin filaments at 4.01s. By comparing the two fluorescent images, it is obvious to see that the image after processing is much clear than the image before processing.